What do platelets look like under a microscope Fenrir / 26.02.202126.02.2021 Platelets under the microscope Look up in the top right quadrant, you can see a smaller purple thing and a larger one. The small one is a regular sized platelet and then the larger one we call that a large platelet- naturally. Now if they get bigger like the size of the Red Blood Cells (pinkish cells) in the background. Platelets under the microscope. Thursday 24th May I am interested in how platelets, the smallest blood cells, function. Platelets are important to prevent bleeding, and a transfusion of platelets can be life-saving for patients with cancer or those who are having surgery. I was watching the magic school bus the other day with my daughter. It was the co where the Ms. Frizzle and the class go into the human body. As a kid I loved the part where they are riding the bloodstream with the visible blood cells. As an adult it gave me the idea to take a look at blood under my own microscope, after I cut my finger in the kitchen. It turns out that it is a very different wwhat what do platelets look like under a microscope you are looking at your own blood cells and seeing them move and change shape right in front of your eyes. In my case I already had a cut on my finger, but I would not suggest cutting yourself for this experiment. You can then safely and cleanly prick your finger to draw an extremely hnder amount of blood. A drop of blood is all you need. Please be sure to read and follow the medical directions and best lile described on the box. Once you have mounted the slide, which we will go over in the next section, looi standard medical treatment to your wound site and ensure you have properly cleaned and bandaged the wound to prevent infection. You can do this by landing the droplet of blood directly on the slide. When you place the blood droplet directly on the slide from your finger, make sure you micrkscope not touch your finger to the slide, only the blood. Otherwise you will get fingerprints on the slide which will reduce the whaat of the image. Next I would recommend adding a droplet of water on the blood sample so that you can see more of the blood cell movement. Loook you do not add water, the blood cells are usually quite close together and you will not get a platflets good image. Now we need to cover the sample with a slide coverslip. To add the slide coverslip, you can use your fingers but if you have a set of tweezers handy, I would recommend using ynder to avoid fingerprints on the coverslip. Drop one side of the coverslip over the sample and then slowly lower the other side of the coverslip. If you drop the coverslip over the sample, you will most likely get air bubbles that will interfere with a clear image. The next step what is a 813 area code to lower lok stage to the lowest level using the coarse focus adjustment knobs and place the slide over the condenser lens. Engage the stage clip and we are ready to go. To see the distinctive red blood cell disk shape, you need a little bit of contrast. I did this by lowering the condenser lens and closing the iris diaphragm to match the 0. I did not undr darkfield in my particular example but viewing red blood cells under a darkfield configuration is a very popular choice. Also using the phase contrast configuration will give you excellent contrast to see the disk shape of the red blood cell. However I recently purchased this DSLR camera and hooked it up to microwcope trinocular mound using this adapter and it takes much better pictures and video. I was lile to the see the disk shape under brightfield so I did not opt for these configurations but if you are having trouble seeing the disk shape you may want to try these options. I was not able to identify platelet cells, which are much smaller than red blood cells. I also was not able to identify white blood cells. This is either because I missed them or because they are less common in any given sample of blood. Blood is a liquid made of mostly of plasma and cells but also contains protein which makes it thicker than water. A little over what do platelets look like under a microscope of the composition of blood is plasma. Plasma is mostly water but there are other components like electrolytes, vitamins, glucose, and proteins that make up a whar percentage of ;latelets. White bloods cells detect and actually eat how to replace garage door extension spring destroy harmful organisms with digestive enzymes. The primary function of platelets is clotting when the body experiences a wound. The platelets pile on top of one another at the site of a wound and with the help of some red what is a community health assessment cells joining the pile they eventually clot the wound enabling the body to ujder. During the healing wat some what do platelets look like under a microscope the platelets fall away and eventually when the wound is fully healed, the remainder of the loo fall away from the site where the what do platelets look like under a microscope once was. Red blood cells are responsible for transporting oxygen throughout the body. It also picks up carbon dioxide and transports it to the lungs for exhalation from the body. Red blood cells are the most common cells found in unver and is part of what plattelets blood the red color. This is primarily because it is the most common cell found in any given sample of blood. A red blood cell also called an erythrocyte, are disk shaped cells with a slightly concaved core. How to turn off hot water supply in house to a donut except there is no hole. Red blood cells are about 6 micrometers in diameter and 2 micrometers thick making them one of the smallest cells in the entire body. Red Blood cells are made primarily of hemoglobin molecules which binds to oxygen and is how red blood cells carry oxygen throughout the body. Red blood cells can maneuver and fit through some of the smallest veins and capillaries in the human body which we saw when we observed the red microsco;e cells floating along with the water. Although we call them cells they cannot replicate and do not have their own nucleus, or DNA. They also cannot express genes and synthesize proteins. Red blood cells only have a life span of about 4 months, so your bone marrow is constantly producing new red blood cells to replace the old ones. This was a pretty cool little lab. It puts things in perspective when you realize that there were millions of red blood cells in that one drop of blood and our body contains around 1. Brandon is an enthusiast, hobbyist, and amateur in the world of microscopy. His love for science and all things microscopic moves him to share everything he knows about microscopy and microbiology. Spirogyra are intricate and beautiful under a microscope. Check out this post to learn more about them! Nematodes are such interesting organisms to observe under the microscope. Check out this post to learn more! Skip to content. Continue Reading. Platelets under the microscope Platelets, the smallest of our blood cells, can only be seen under a microscope. They’re literally shaped like small plates in their non-active form. A blood vessel will send out a signal when it becomes damaged. When platelets receive that signal, they’ll respond by traveling to the area and transforming into their “active” formation. Best Way to Observe Blood Under the Microscope. To see the distinctive red blood cell disk shape, you need a little bit of contrast. I did this by lowering the condenser lens and closing the iris diaphragm to match the numerical aperture of the 40X objective I was using. What do platelets look like under a microscope? debris. What are the major structural elements of erythrocytes? 1. Small 2. Biconcave shape (flattened discs with depressed centers) 3. Lighter in color at their thin centers 4. Have no nucleus 5. No organelles 6. Are "bags" of hemoglobin. Platelets are produced in the bone marrow, the same as the red cells and most of the white blood cells. Platelets are produced from very large bone marrow cells called megakaryocytes. As megakaryocytes develop into giant cells, they undergo a process of fragmentation that results in the release of over 1, platelets per megakaryocyte. The dominant hormone controlling megakaryocyte development is thrombopoietin often abbreviated as TPO. Platelet Structure. Platelets are actually not true cells but merely circulating fragments of cells. But even though platelets are merely cell fragments, they contain many structures that are critical to stop bleeding. They contain proteins on their surface that allow them to stick to breaks in the blood vessel wall and also to stick to each other. They contain granules that can secrete other proteins required for creating a firm plug to seal blood vessel breaks. Also platelets contain proteins similar to muscle proteins that allow them to change shape when they become sticky. The pictures above show normal platelets on the left. They are shaped like a plate, therefore their name. When platelets are stimulated by a break in the blood vessel wall they change shape as shown in the other three pictures. They become round and extend long filaments. They may even look like an octopus, with long tentacles reaching out to make contact with the broken blood vessel wall or with other platelets. With these long filaments, platelets then form a plug to seal the broken blood vessel. In addition to being the smallest blood cell, platelets are also the lightest. Therefore they are pushed out from the center of flowing blood to the wall of the blood vessel. There they roll along the surface of the vessel wall, which is lined by cells called endothelium. The endothelium is a very special surface, like Teflon, that prevents anything from sticking to it. However when there is an injury or cut, and the endothelial layer is broken, the tough fibers that surround a blood vessel are exposed to the liquid flowing blood. It is the platelets that react first to injury. The tough fibers surrounding the vessel wall, like an envelop, attract platelets like a magnet, stimulate the shape change that is shown in the pictures above, and platelets then clump onto these fibers, providing the initial seal to prevent bleeding, the leak of red blood cells and plasma through the vessel injury. The color photograph is a microscopic picture of a drop of blood spread out onto a glass slide. The magnification is not as high as the pictures above, so the platelets seem very small. It can be seen that as the platelets touch the glass, they begin to stick together forming a long string. This illustrates the basic function of platelets, to stick to any foreign surface and then to stick together. The red blood cells in this picture are normal, with their round shape and their thin center. Disorders of Platelet Function. The most common disorder of platelet function is caused by aspirin. Aspirin blocks one of the steps required for platelets to stick together. This effect of aspirin is what makes it an effective treatment for patients who have disorders of blood clotting or thrombosis. For example, a person coming to an emergency room with severe chest pain and a suspected heart attack is immediately given aspirin. This prevents some of the platelet clumping which can obstruct the blood flow to the heart. Aspirin is an effective medicine to prevent these clots, but it does not totally paralyze the platelet. Therefore many people take daily aspirin and have no problems with bleeding. However aspirin can be potentially dangerous in patients who already have a risk for bleeding, such as boys with hemophilia or patients who have too few platelets and depend on each platelet having full function. Rare conditions result in the bone marrow producing too many platelets, sometimes as many as one million or two million per microliter. In some of these patients, there are increased risks for blood clots, but many patients with these disorders have no problems. Thrombocytopenia is the principal focus of this website. Thrombocytopenia can be caused by failure of the bone marrow to produce normal numbers of platelets. Bone marrow failure has multiple causes. These are not discussed on this website. Thrombocytopenia can also be caused by increased destruction of platelets once they are produced and released into the circulating blood. These disorders are the focus of this website. They are described briefly here and in more detail in their specific sections on this website. Immune Thrombocytopenia ITP - This disorder, described in its own section on this website, is caused both by increased platelet destruction and also decreased bone marrow platelet production. These problems are caused by autoantibodies. Antibodies are proteins normally made by a type of white blood cells to react with and defend against foreign materials. For example, antibodies are normally formed to bacteria and viruses, and help with the healing process. Antibodies are normally stimulated by immunization and vaccination, to prevent infections. Antibodies normally react with organ transplants and attempt to reject these transplants. Autoantibodies are abnormal. These occur when antibody-producing cells receive mixed signals, and identify a normal body tissue as foreign and try to reject it. The term, autoantibody, means an antibody that reacts with the person herself, not foreign material. The autoantibodies destroy the platelets rapidly after they are produced. The autoantibodies also react with the bone marrow megakaryocytes and inhibit platelet production. The information included in this website is for educational purposes only. The designers and operators of this site take no responsibility for the things you may do with this information. For advice on your unique medical condition, please consult your health care professional. By going further into this website you acknowledge that you have read and understood this disclaimer. Menu: Home. Thrombotic Microangiopathy. Complement-mediated TMA. Platelets In this section we introduce platelets themselves. Platelets are the smallest of the three major types of blood cells. The normal platelet count is ,, per microliter of blood, but since platelets are so small, they make up just a tiny fraction of the blood volume. The principal function of platelets is to prevent bleeding. Red blood cells are the most numerous blood cell, about 5,, per microliter. Their color is caused by hemoglobin, which accounts for nearly all of the red cell volume. Hemoglobin is the critical protein that transports oxygen from our lungs to the tissues. Red cells are normally shaped as round, biconcave discs. With microscopic examination, they look like a red or orange tire with a thin, almost transparent center. White blood cells are the largest of the blood cells but also the fewest. There are only 5, to 10, white blood cells per microliter. There are several different types of white cells but all are related to immunity and fighting infection. Platelet Production Platelets are produced in the bone marrow, the same as the red cells and most of the white blood cells. Platelet Structure Platelets are actually not true cells but merely circulating fragments of cells. Platelet Function In addition to being the smallest blood cell, platelets are also the lightest. Disorders of Platelet Function The most common disorder of platelet function is caused by aspirin. Disorders of Platelets Number: Too Many Platelets Rare conditions result in the bone marrow producing too many platelets, sometimes as many as one million or two million per microliter. Almost all medications can cause bad allergic reactions in sensitive people, but these reactions are rare. Most commonly, drugs may cause a red rash in sensitive people. Drugs can also cause serious reactions with blood platelets. In these reactions, drugs stick to the platelet surface, and this combination of the drug bound to the platelet can be recognized by the body as a foreign substance and the body then makes an antibody to the drug-coated platelets, and all platelets can be destroyed. When the drug is stopped, platelet destruction is stopped, and the platelet count returns to normal. But these drug-dependent antibodies can persist for many years, the same way that protective antibodies persist for many years after immunizations. So if the patient takes the drug again, the platelets are coated, the antibody reacts with the platelets and again the platelet count falls immediately to low levels. Therefore recognition of a drug as the cause for thrombocytopenia is critical to avoid any further exposure to that drug. This is described in its own section on this website. The platelet count is not very low. Our opinion is that this is merely a normal adjustment of the platelet count during pregnancy, that the platelet counts of pregnant women, particularly near the end of pregnancy and at the time of delivery, all shift down a little bit. A reason for this is that the plasma volume is increased during pregnancy, and therefore the platelets are simply diluted in the larger volume of plasma. This is the explanation for why the blood hemoglobin concentration is also a little lower during pregnancy. Our opinion is that this is not a health problem and it does not require any additional testing or care. Thrombotic microangiopathies TMA - These disorders are the result of abnormal blood clotting in the smallest blood vessels arterioles, capillaries throughout all of the body. There are multiple causes of these syndromes. All are uncommon. The most important of these syndromes, thrombotic thrombocytopenic purpura, hemolytic uremic syndrome, drug-induced TMA, and complement-mediated TMA, are described in their own sections on this website.